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anti epcam pe  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec anti epcam pe
    Anti Epcam Pe, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 83 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd326+epcam/CD326+(EpCAM)+Antibody%2C+anti-human%2C+REAfinity/pmc13421491-77-45-48
    Average 95 stars, based on 83 article reviews
    anti epcam pe - by Bioz Stars, 2026-10
    95/100 stars

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    Related Articles

    Expressing:

    Article Title: Establishment, culture and characterization of gemcitabine hydrochloride‐resistant human non‐small cell lung carcinoma cell line derived cancer stem cells
    Article Snippet: Cellular Therapy and Stem Cell Production Application and Research Centre, ESTEM, Eskisehir Osmangazi University, Eskisehir, Turkey Department of Stem Cell, Eskisehir Osmangazi University, Eskisehir, Turkey Department of Medical Laboratory Techniques, Vocational School of Health Services, Eskisehir Osmangazi University, Eskisehir, Turkey Department of Metallurgical and Materials Engineering, Engineering and Architecture Faculty, Eskisehir Osmangazi University, Eskisehir, Turkey Translational Medicine Research and Clinical Center, TATUM, Eskisehir Osmangazi University, Eskisehir, Turkey Department of Medicinal Oncology, Faculty of Medicine, Eskisehir Osmangazi University, Eskisehir, Turkey Department of Emergency Medicine, Ministry of Health Eskişehir City Hospital, Eskisehir, Turkey

    Cell Isolation:

    Article Title: Establishment, culture and characterization of gemcitabine hydrochloride‐resistant human non‐small cell lung carcinoma cell line derived cancer stem cells
    Article Snippet: Cellular Therapy and Stem Cell Production Application and Research Centre, ESTEM, Eskisehir Osmangazi University, Eskisehir, Turkey Department of Stem Cell, Eskisehir Osmangazi University, Eskisehir, Turkey Department of Medical Laboratory Techniques, Vocational School of Health Services, Eskisehir Osmangazi University, Eskisehir, Turkey Department of Metallurgical and Materials Engineering, Engineering and Architecture Faculty, Eskisehir Osmangazi University, Eskisehir, Turkey Translational Medicine Research and Clinical Center, TATUM, Eskisehir Osmangazi University, Eskisehir, Turkey Department of Medicinal Oncology, Faculty of Medicine, Eskisehir Osmangazi University, Eskisehir, Turkey Department of Emergency Medicine, Ministry of Health Eskişehir City Hospital, Eskisehir, Turkey

    Flow Cytometry:

    Article Title: Establishment, culture and characterization of gemcitabine hydrochloride‐resistant human non‐small cell lung carcinoma cell line derived cancer stem cells
    Article Snippet: Cellular Therapy and Stem Cell Production Application and Research Centre, ESTEM, Eskisehir Osmangazi University, Eskisehir, Turkey Department of Stem Cell, Eskisehir Osmangazi University, Eskisehir, Turkey Department of Medical Laboratory Techniques, Vocational School of Health Services, Eskisehir Osmangazi University, Eskisehir, Turkey Department of Metallurgical and Materials Engineering, Engineering and Architecture Faculty, Eskisehir Osmangazi University, Eskisehir, Turkey Translational Medicine Research and Clinical Center, TATUM, Eskisehir Osmangazi University, Eskisehir, Turkey Department of Medicinal Oncology, Faculty of Medicine, Eskisehir Osmangazi University, Eskisehir, Turkey Department of Emergency Medicine, Ministry of Health Eskişehir City Hospital, Eskisehir, Turkey

    Staining:

    Article Title: Epithelial cells maintain memory of prior infection with Streptococcus pneumoniae through di-methylation of histone H3
    Article Snippet: To purify epithelial cells, lungs homogenate was filtered through 100 μm stainer (Miltenyi Biotec, 130-098-463), and samples were centrifuge for 10 min at 200 × g . Cell pellets were suspended in 1 mL RBC lysis buffer 1X (Biolegend; 43031) and incubated for 3 min at room temperature to lyse remaining RBC. .. Cell pellets were washed with 20 mL PBS/FCS 1%, and samples were centrifuged for 10 min at 200 × g . Finally, the cell pellet was resuspended in 10 mL PBS/FCS 1%, filtered through 40 mm stainer (greiner Bio-one, 542040), and centrifuge 10 min at 200 × g . The cell pellet was resuspended in PBS/FCS 1% and the cells were stained with CD45.1 Monoclonal PE (0.1 μg; eBioscience TM ; 12045383) and CD326 (EpCam) APC (1:50; Miltenyi Biotec; 130-117-865) antibodies and incubated for 1 h at 4 °C. .. Samples were washed with 3 mL PBS/FCS 1% and centrifuged for 10 min at 200 g. Samples were then stained with Fixable Viability dye eFluor TM 780 1:1000 (eBioscience TM , 65-0865-14) for 5 min at 4 °C, in the dark and washed with 3 mL PBS/FCS 1% and centrifuge for 10 min at 200 × g . Samples were Fixed in PFA 4% for 10 min at RT in the dark washed with 3 mL PBS/FCS 1%, and centrifuge for 10 min at 200 × g . Fixed cells were stained with H3K4me2 antibody (1:1000; Abcam ab32356), Isotype control Rabbit IgG monoclonal (Abcam; ab172730), Alexa Fluor 488 secondary antibody (1:1000; Invitrogen TM A11034) using the protocol previously described in the immunofluorescence section.

    Article Title: Epithelial cells maintain memory of prior infection with Streptococcus pneumoniae through di-methylation of histone H3.
    Article Snippet: To purify epithelial cells, lungs homogenate was filtered through 100μm stainer (Miltenyi Biotec, 130-098-463), and samples were centrifuge for 10min at 200 × g. Cell pellets were suspended in 1mL RBC lysis buffer 1X (Biolegend; 43031) and incubated for 3min at room temperature to lyse remaining RBC. .. Cell pellets were washed with 20mL PBS/FCS 1%, and samples were centrifuged for 10min at 200× g. Finally, the cell pellet was resuspended in 10mL PBS/FCS 1%, filtered through 40mm stainer (greiner Bio-one, 542040), and centrifuge 10min at 200× g. The cell pellet was resuspended in PBS/FCS 1% and the cells were stained with CD45.1 Monoclonal PE (0.1μg; eBioscienceTM; 12045383) and CD326 (EpCam) APC (1:50; Miltenyi Biotec; 130-117-865) antibodies and incubated for 1 h at 4 °C. ..

    Incubation:

    Article Title: Epithelial cells maintain memory of prior infection with Streptococcus pneumoniae through di-methylation of histone H3
    Article Snippet: To purify epithelial cells, lungs homogenate was filtered through 100 μm stainer (Miltenyi Biotec, 130-098-463), and samples were centrifuge for 10 min at 200 × g . Cell pellets were suspended in 1 mL RBC lysis buffer 1X (Biolegend; 43031) and incubated for 3 min at room temperature to lyse remaining RBC. .. Cell pellets were washed with 20 mL PBS/FCS 1%, and samples were centrifuged for 10 min at 200 × g . Finally, the cell pellet was resuspended in 10 mL PBS/FCS 1%, filtered through 40 mm stainer (greiner Bio-one, 542040), and centrifuge 10 min at 200 × g . The cell pellet was resuspended in PBS/FCS 1% and the cells were stained with CD45.1 Monoclonal PE (0.1 μg; eBioscience TM ; 12045383) and CD326 (EpCam) APC (1:50; Miltenyi Biotec; 130-117-865) antibodies and incubated for 1 h at 4 °C. .. Samples were washed with 3 mL PBS/FCS 1% and centrifuged for 10 min at 200 g. Samples were then stained with Fixable Viability dye eFluor TM 780 1:1000 (eBioscience TM , 65-0865-14) for 5 min at 4 °C, in the dark and washed with 3 mL PBS/FCS 1% and centrifuge for 10 min at 200 × g . Samples were Fixed in PFA 4% for 10 min at RT in the dark washed with 3 mL PBS/FCS 1%, and centrifuge for 10 min at 200 × g . Fixed cells were stained with H3K4me2 antibody (1:1000; Abcam ab32356), Isotype control Rabbit IgG monoclonal (Abcam; ab172730), Alexa Fluor 488 secondary antibody (1:1000; Invitrogen TM A11034) using the protocol previously described in the immunofluorescence section.

    Article Title: Epithelial cells maintain memory of prior infection with Streptococcus pneumoniae through di-methylation of histone H3.
    Article Snippet: To purify epithelial cells, lungs homogenate was filtered through 100μm stainer (Miltenyi Biotec, 130-098-463), and samples were centrifuge for 10min at 200 × g. Cell pellets were suspended in 1mL RBC lysis buffer 1X (Biolegend; 43031) and incubated for 3min at room temperature to lyse remaining RBC. .. Cell pellets were washed with 20mL PBS/FCS 1%, and samples were centrifuged for 10min at 200× g. Finally, the cell pellet was resuspended in 10mL PBS/FCS 1%, filtered through 40mm stainer (greiner Bio-one, 542040), and centrifuge 10min at 200× g. The cell pellet was resuspended in PBS/FCS 1% and the cells were stained with CD45.1 Monoclonal PE (0.1μg; eBioscienceTM; 12045383) and CD326 (EpCam) APC (1:50; Miltenyi Biotec; 130-117-865) antibodies and incubated for 1 h at 4 °C. ..

    other:

    Article Title: Guidelines for preparation and flow cytometry analysis of human nonlymphoid tissue DC
    Article Snippet: VioBlue , CD326(Epcam) , HEA‐125 , 130‐113‐266 , Miltenyi Biotec , 3:150.



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